Zeiss Light Sheet 7
Light Sheet Fluorescence Microscopy (LSFM) is an extremely powerful alternative to
established fluorescence imaging techniques, especially for 3-D imaging within whole
live organisms and large tissue explants. Ideal for fast and gentle imaging of whole
living model organisms, tissues, and cells as they develop – over extended periods
of time capable of imaging large optically cleared specimens in toto – with subcellular
resolution. By selectively illuminating the observed optical section with a thin sheet
of light, photo bleaching is reduced to a minimum, making light sheet microscopy ideal
for nondestructive imaging of fragile samples over extended periods of time. Equipped
with an incubation system with temperature and CO2 control for long-term in vivo experiments.
Features and Specifications
Objectives
Magnification | Objective Type | Description | NA | Immersion | WD (mm) |
---|---|---|---|---|---|
2.5 | Fluar | 2.5x | 0.12 |
Clearing |
8.7 |
5 | EC Plan-Neofluar | 5x | 0.16 foc | Water/Clearing | 18.5 |
5 | EC Plan-Neofluar | 5x | 0.16 | Clearing | 18.5 |
10 | W Plan-Apochromat |
10x |
0.50 | Water | 3.7 |
20 | W Plan-Apochromat | 20x | 1.00 | Water | 2.4 |
20 | W Plan-Apochromat | 20x | 1.00 | Clearing | 6.4 |
Lasers lines wavelengths (nm)
405, 445, 488, 514, 561, 638
Camera
Two liquid cooled sCMOS cameras, pco.EDGE
Filter Sets
Fluorochromes | Mirror | Filter 1 (nm) | Filter 2 (nm) |
---|---|---|---|
DAPI/GFP | SBS LP 490 | BP 420-470 (DAPI) | BP 505-545 (GFP) |
DAPI/RFP | SBS LP 510 | BP 420-470 (DAPI) | BP 505-545 (GFP) |
GFP/mCherry | SBS LP 560 | BP 505-545 (GFP) | LP 585 (mCherry) |
GFP/ Draq5(toto3) | SBS LP 560 | BP 505-545 (GFP) | LP 660 (Draq5(toto3)) |
RFP/ Draq5(toto3) | SBS LP 640 | BP 575-615 (RFP) | LP 660 (Draq5(toto3)) |
Available Capillary Tubes
Color | Diameter (mm) |
---|---|
Red | 0.68 |
Black | 1.00 |
Green | 1.50 |
Blue | 2.15 |